Improved Method for n-Alkanes and n-Methyl-Ketones Determination in Litters and Soils

Autor: L. G. Bogatyrev, Yu. A. Zavgorodnyaya, N. A. Anokhina, Vladimir V. Demin
Rok vydání: 2020
Předmět:
Zdroj: Eurasian Soil Science. 53:310-316
ISSN: 1556-195X
1064-2293
DOI: 10.1134/s1064229320030114
Popis: A new technique to extract lipid biomarkers (n-alkanes and n-methyl-ketones) from forest litters and soils using the accelerated solvent extraction (ASE) method was developed. The maximum output of n-alkanes and n-methyl-ketones was obtained by sequential extraction using the chloroform : methanol mixture [3 : 1 (vol)] in the first two extraction cycles and chloroform in the subsequent two cycles. The extraction has been carried out at the temperature of 100°С, pressure of 10.3 MPa, and static extraction time of 5 min. The repeated chloroform treatment by ASE was shown to increase the output of long-chain n-alkanes and n-methyl-ketones.
Databáze: OpenAIRE
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