Non-Processive Force Generation by Mammalian Axonemal Dynein In Situ on Doublet Microtubules

Autor:	Kathleen A. Lesich, David P. Lorch, Alan J. Hunt, Charles B. Lindemann
Rok vydání:	2013
Předmět:	Axoneme Nexin Cilium Dynein macromolecular substances Biology Flagellum General Biochemistry Genetics and Molecular Biology Cell biology Optical tweezers Microtubule Modeling and Simulation Dynactin biology.protein
Zdroj:	Cellular and Molecular Bioengineering. 6:431-440
ISSN:	1865-5033 1865-5025
DOI:	10.1007/s12195-013-0310-y
Popis:	We utilize optical tweezers to examine displacements produced by small numbers of dynein motors located in situ on doublet microtubules from disintegrated mammalian sperm axonemes. In contrast with cytoplasmic dynein, we find that axonemal dynein is not processive, and the duration of individual force-generating interactions with a microtubule are longer than predicted from the velocity of movements generated by large ensembles of motors. These findings suggest that tension is required for rapid release of dynein following a power stroke and may explain how axonemal dynein is adapted to work in arrays within an axoneme, where cyclical bending patterns require motors to function over a range of sliding velocities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1972e724c91822a0d5145d3030a67688 https://doi.org/10.1007/s12195-013-0310-y Zobrazit plný text záznamu Full text from SpringerLink