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Publisher Summary This chapter focuses on molecular cytogenetic methodologies and a bacterial artificial chromosome (BAC) probe panel resource for genomic analyses in zebrafish. The zebrafish genome contains 1.7 × 10 9 bp of DNA, approximately half the genome size of most mammals, organized into 25 pairs of chromosomes (2 n = 50). Classical cytogenetic studies of zebrafish have shown that individual chromosomes are difficult to unequivocally identify on the basis of chromosome size, morphology (i.e., position of the centromere), and banding pattern. Because cytogenetics offers a unique perspective in genome analysis, by directly visualizing targeted chromosomal loci and large genomic alterations, it is important to advance zebrafish cytogenetics and develop the resources that may provide valuable complementary methods for genetic analyses in this important model organism. The molecular cytogenetic methods described in this chapter provide complementary assays for genetic analysis of zebrafish mutants. In addition, the development of a BAC probe panel resource provides an initial cytogenetic framework for the accurate assembly of the zebrafish genome sequence that is independent of existing genetic and radiation hybrid maps. |
