Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay
| Autor: | Zhi Min Ren, Sheng Shu Ai, Xi Nie |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Materials science Chromatography medicine.diagnostic_test biology Polyvinylpyrrolidone Blocking (radio) General Engineering Polymer Microbead (research) Polyvinyl alcohol chemistry.chemical_compound chemistry Immunoassay medicine biology.protein Polystyrene Bovine serum albumin medicine.drug |
| Zdroj: | Advanced Materials Research. :858-861 |
| ISSN: | 1662-8985 |
| DOI: | 10.4028/www.scientific.net/amr.641-642.858 |
| Popis: | In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|