| Autor: |
Kyung-Hun Park, Are-Sun You, Mi-Kyoung Kim, Jae-Eup Park, Soo-Jin Park, Soon-Sung Hong, Mi-Hye Jeong |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
The Korean Journal of Pesticide Science. 16:261-266 |
| ISSN: |
1226-6183 |
| DOI: |
10.7585/kjps.2012.16.3.261 |
| Popis: |
It is common to use many experiment animals to evaluate the toxicity of chemicals including pesticides. For protecting animal, the concepts of 3R (Reduction, Replacement, Refinement) were introduced and in vitro alternatives methods actively have been developed all over the world. Many experimental animals for toxicological tests have been used, so that it is important to establish the alternative methods. In this study, the alternative method using reconstituted human skin model (Keraskin TM ) was conducted for classification of skin irritation on pesticides. Sixteen formulations selected on the basis of the degree of irritation were treated by Keraskin TM test. The percent of cell viability was measured into the culture medium collected after treatment of the pesticides for 24-72 hrs. The skin irritations of formulations were evaluated by the cell viability. In this study, The 4 formulations with mild irritation in rabbits were evaluated as non- irritant, the 6 formulations with moderate and severe irritation were evaluated as irritant in human skin model test. We suggest that the alternative test using Keraskin TM model could be used as toxicity evaluation for primary irritation index (P.I.I.) score of greater than or equal to 2.1 of pesticides. The further studies should be required to apply for hazardous assessment of pesticides on alternative skin irritation methods because of the interindividual variability of the sensitivity of skin irritation on pesticides. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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