| Popis: |
N-2-acetylaminofluorene (AAF) is a potential liver carcinogen, which after metabolic activation binds to liver DNA, RNA and proteins when administered in vivo. N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF), a metabolite of AAF, reacts in vitro with DNA yielding two adducts; one of them, N9-deoxyguanosine-8-y1)-AAF represents about 80% of the modified DNA (Miller et al., 1966). It has been shown that AAF bound to mature DNA induces a conformational change in the vicinity of the modified site leading to insertion of the fluorene ring between adjacent base pairs and producing local denaturation of the double helix (Fuchs and Daune, 1972). |
