Synthesis and Localization of Pancreatic Secretory Proteins in Pancreatic Acinar-like Metaplasia in the Distal Part of the Oesophagus
| Autor: | A Borgström, Hans-Olof Håkansson, Johan Johansson, Lennart Mellblom, A Bjartell |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Pancreatic acinar metaplasia Pathology Trypsinogen Gastroenterology Biology medicine.disease digestive system digestive system diseases chemistry.chemical_compound medicine.anatomical_structure Secretory protein Endocrinology Intestinal mucosa chemistry Internal medicine Metaplasia medicine medicine.symptom Pancreas Pancreatic elastase Pancreatic Secretory Trypsin Inhibitor |
| Zdroj: | Scandinavian Journal of Gastroenterology. 38:10-13 |
| ISSN: | 1502-7708 0036-5521 |
| Popis: | Background Pancreatic acinar-like metaplasia has previously been described in the gastric mucosa and in the distal part of the oesophagus. The resemblance to pancreatic acinar cells prompted us to study the possible occurrence of secretory pancreatic proteins in these cells. Methods Seven specimens obtained from the distal oesophagus at gastroscopy where routine microscopy showed pancreatic acinar-like metaplasia were selected for this study. Sections were subjected to immunohistochemical detection of trypsinogen, pancreatic elastase, procarboxypeptidase B and pancreatic secretory trypsin inhibitor using specific antisera. An alkaline-phosphatase-conjugated oligodeoxynucleotide probe, complementary to the transcript for trypsinogen 2 (anionic) was used for in situ hybridization. Results Cells with pancreatic acinar-like metaplasia were immunoreactive to all pancreatic secretory proteins studied. In situ hybridization showed the presence of trypsinogen 2 mRNA in pancreatic acinar-like metaplasia. The pancreatic proteins were not seen in other cells in the distal oesophagus. Conclusion Pancreatic acinar-like metaplasia is common in the distal oesophagus and pancreatic secretory proteins, including trypsininogen 2, are produced in the oesophageal metaplastic acinar cells. The biological significance of this finding has yet not been thoroughly studied. |
| Databáze: | OpenAIRE |
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