Improved Stabilities of Lipase by Immobilization on Eupergit C

Autor: Li Su, Quan Fa Zhou, Chun Yong Zhang, De Ma, Bin Wang, Xiu Ying Hu
Rok vydání: 2015
Předmět:
Zdroj: Advanced Materials Research. 1096:219-223
ISSN: 1662-8985
DOI: 10.4028/www.scientific.net/amr.1096.219
Popis: Lipase is frequently used for catalyze wide non-natural substrates in order to obtain enantio- and regioselectivie substrates. This study evaluates the stabilities of lipase by immobilization on Eupergit C. The immobilized lipase had improved stability at 60oC for 60 h compared to the free lipase 1 h at 60oC. Immobilization resulted in an increase in pH stability over a range of 7.0 - 9.0 and about 54 days for half-life of storage at 4oC. The tolerance of lipase to organic solvents was also improved by immobilization, and the immobilized lipase showed activating activity when exposed to hydrophobic solvents.
Databáze: OpenAIRE