Microbial diffraction gratings as optical detectors for heavy metal pollutants

Autor:	David A. Noever, Andrew Brittain, Raymond J. Cronise, Shannon Armstrong, Helen C. Matsos, Don Obenhuber
Rok vydání:	1996
Předmět:	Pollutant Cadmium education.field_of_study biology Population Tetrahymena chemistry.chemical_element Luminous intensity biology.organism_classification Copper chemistry Environmental chemistry Toxicity Tetrahymena pyriformis education Instrumentation
Zdroj:	Review of Scientific Instruments. 67:828-832
ISSN:	1089-7623 0034-6748
DOI:	10.1063/1.1146817
Popis:	As a significant industrial pollutant, cadmium is implicated as the cause of itai‐itai disease. For biological detection of cadmium toxicity, an assay device has been developed using the motile response of the protozoa species, Tetrahymena pyriformis. This mobile protozoa measures 50 μm in diameter, swims at 10 body lengths per second, and aggregates into macroscopically visible patterns at high organism concentrations. The assay demonstrates a Cd+2 sensitivity better than 1 μM and a toxicity threshold to 5 μM, thus encouraging the study of these microbial cultures as viable pollution detectors. Using two‐dimensional diffraction patterns within a Tetrahymena culture, the scattered light intensity varies with different organism densities (population counts). The resulting density profile correlates strongly with the toxic effects at very low dosages for cadmium (
Databáze:	OpenAIRE
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