Microbiological culture and panbacterial PCR yield of diluted or undiluted vitreous from vitrectomy are comparable in acute postsurgery endophthalmitis (FRIENDS group)

Autor: V Vinh Moreau Gaudry, André Pechinot, Max Maurin, Gilles Thuret, Jp Romanet, Cp Creuzot, C. Chiquet, P.L. Cornut, François Vandenesch, P. Denis, Am Bron
Rok vydání: 2008
Předmět:
Zdroj: Acta Ophthalmologica. 86
ISSN: 1755-375X
DOI: 10.1111/j.1755-3768.2008.6326.x
Popis: Purpose To compare the diagnostic yield of microbiological analysis performed on diluted and undiluted vitreous samples from pars plana vitrectomy in patients with acute postcataract surgery endophthalmitis. Methods Undiluted and diluted vitreous samples were harvested in 34 patients at the beginning of the vitrectomy, among the 57 vitrectomized patients (out of 100 patients presenting with an acute postcataract surgery endophthalmitis) included in the multicenter prospective study FRIENDS (2004-2005). Vitrectomy was performed after one (n=12) or two (n=22) antibiotics intravitreal injections. Each vitreous sample was divided into two parts for conventional culture (Brain Heart Infusion broth) and panbacterial PCR, respectively. Results Microbiological analysis of both undiluted and diluted vitreous allowed a bacterial identification in 28 out of 34 cases (82.3%). Microbiological diagnosis using undiluted vitreous and conventional culture was negative in 32 cases and positive in 2 cases. In one case, there was a discrepancy between undiluted (culture negative) and diluted vitreous (S. epidermidis). Analysis of both undiluted and diluted vitreous using PCR was positive in 28 cases and negative in 6/34 cases. There was a discrepancy between both samples in three cases. Conclusion Microbiological results combining PCR and cultures showed that diluted vitreous analysis may replace analysis of undiluted vitreous. The diluted sampling of vitreous, more easily performed and less iatrogenic, may be recommended when panbacterial PCR is used .
Databáze: OpenAIRE
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