LOCALIZATION OF AMYLOSE AND AN AMYLOSE-BINDING LIPOPROTEIN IN NEUROSPORA CRASSA HYPHAE BY CYTOCHEMISTRY AND IMMUNOELECTRON MICROSCOPY

Autor:	Jacob P. Varkey, Derek A. McCracken, Mathew J. Nadakavukaren
Rok vydání:	1985
Předmět:	biology Physiology Immunoelectron microscopy fungi Plant Science biology.organism_classification Neurospora Silver proteinate Neurospora crassa Cell wall chemistry.chemical_compound Biochemistry chemistry Amylose Cytochemistry Ultrastructure
Zdroj:	New Phytologist. 101:623-630
ISSN:	1469-8137 0028-646X
DOI:	10.1111/j.1469-8137.1985.tb02868.x
Popis:	Summary The presence of amylose was demonstrated in hyphal walls of Neurospora crassa Shen and Dodge by silver proteinate staining for carbohydrates and differential digestion with β-amylase. The amylose was found to be localized in the innermost layer of the wall. Similarly, amylose-precipitating factor (APF) was also found to be localized in the same wall layer using ferritin-tagged antibodies to APF. Lipase-digestion of the isolated hyphal wall resulted in considerable reduction of the silver grain deposition in the innermost wall layer, suggesting the removal of amylose from this layer. Because lipid-free APF cannot bind to amylose, these observations indicate that APF and amylose in Neurospora are in a complexed form in the innermost layer of the hyphal wall as a structural component.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::190ef073890716665f6e4a15ed169fbc https://doi.org/10.1111/j.1469-8137.1985.tb02868.x Zobrazit plný text záznamu Plný text