A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)
| Autor: | Senem Kiralp Kayahan, Özlem Türkarslan, Levent Toppare |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Conductive polymer
Chromatography Cholesterol oxidase Cholesterol Metals and Alloys Condensed Matter Physics Electrochemistry Substrate concentration Amperometry Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound chemistry Materials Chemistry Electrical and Electronic Engineering Instrumentation Enzyme Electrodes Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 136:484-488 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2008.10.016 |
| Popis: | Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. Km, Imax and sensitivity (Imax/Km) were calculated as 3.4 mM, 34 μA cm−2 and 10 μA mM−1 cm−2, respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity. |
| Databáze: | OpenAIRE |
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