Synthetic Biology Toolkits and Metabolic Engineering Applied in Corynebacterium glutamicum for Biomanufacturing
| Autor: | Xueqin Lv, Meng Chai, Deng Chen, Wei Lu, Long Liu, Yanfeng Liu, Qi Chen, Jianghua Li, Guocheng Du |
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| Rok vydání: | 2021 |
| Předmět: |
Engineering
business.industry Biomedical Engineering General Medicine Directed evolution Biochemistry Genetics and Molecular Biology (miscellaneous) Corynebacterium glutamicum Metabolic engineering Synthetic biology Bioproducts bacteria Biomanufacturing Biochemical engineering business Flux (metabolism) |
| Zdroj: | ACS Synthetic Biology. 10:3237-3250 |
| ISSN: | 2161-5063 |
| Popis: | Corynebacterium glutamicum is an important workhorse in industrial white biotechnology. It has been widely applied in the producing processes of amino acids, fuels, and diverse value-added chemicals. With the continuous disclosure of genetic regulation mechanisms, various strategies and technologies of synthetic biology were used to design and construct C. glutamicum cells for biomanufacturing and bioremediation. This study mainly aimed to summarize the design and construction strategies of C. glutamicum-engineered strains, which were based on genomic modification, synthetic biological device-assisted metabolic flux optimization, and directed evolution-based engineering. Then, taking two important bioproducts (N-acetylglucosamine and hyaluronic acid) as examples, the applications of C. glutamicum cell factories were introduced. Finally, we discussed the current challenges and future development trends of C. glutamicum-engineered strain construction. |
| Databáze: | OpenAIRE |
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