EVALUATION OF MICRORNAS RELATED TO THE SONIC HEDGEHOG PATHWAY IN ORAL CANCER
| Autor: | Rômulo Sperduto Dezonne, Simone De Queiroz Chaves Lourenço, Tania Cristina Leite de Sampaio e Spohr, Silvia Maria De Carvalho Lyra, Vivaldo Moura-Neto, Cláudia Pereira, Andréa Braga Moleri |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.disease_cause
Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine GLI1 microRNA Medicine Radiology Nuclear Medicine and imaging Dentistry (miscellaneous) Sonic hedgehog biology business.industry Cancer 030206 dentistry medicine.disease Hedgehog signaling pathway stomatognathic diseases Real-time polymerase chain reaction 030220 oncology & carcinogenesis biology.protein Cancer research Immunohistochemistry Surgery Oral Surgery business Carcinogenesis |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 129:e133 |
| ISSN: | 2212-4403 |
| DOI: | 10.1016/j.oooo.2019.06.582 |
| Popis: | The microRNAs (miRNAs) as biomarkers with clinical application in oral squamous cell carcinoma (OSCC) has been suggested in literature. These small noncoding RNA molecules can act as oncogenes or tumor suppressor molecules. Altered expression of miR-17, miR-214, and miR-324 was described and related to the sonic hedgehog (SHH) pathway in medulloblastomas. SHH is an important embryonic pathway associated with progression and poor prognosis in OSCC. A better comprehension about the miRNA activity will improve the knowledge about the SHH pathway in oral carcinogenesis. Objective: This study aims to evaluate the Gli1 (an important SHH component) and miR-17, miR-214, and miR-324 expression in OSCC. Study Design: Gli1 evaluation was performed by immunohistochemistry and miR-17, miR-214, and miR-314 expression was investigated using real time polymerase chain reaction in 20 OSCC samples. Results: Gli1 demonstrated a cytoplasmic and nuclear positivity mainly in the undifferentiated histologic grade. Hypoexpression of miR-324 and miR-214 was observed in 40% and 60% of OSCC samples, respectively. A higher expression of miR-17 was identified in 60% of these samples. Conclusion: A possible tumor suppressor and oncogenic profile of miR-324 and miR-17, respectively, as mentioned in medulloblastomas was observed. However, miR-214 did not demonstrate an oncogenic behavior on OSCC. |
| Databáze: | OpenAIRE |
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