Occurrence of Listeria monocytogenes on the seafood contact surfaces of Tuticorin Coast of India
| Autor: | M. Palani Kumar, R. Jeya Shakila, G. Jeyasekaran, D. Sukumar, R. Selvaganapathi, Balasubramanian Sivaraman |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary medicine Fish market Seafood processing 030106 microbiology Pcr assay Fishing food and beverages 04 agricultural and veterinary sciences Biology medicine.disease_cause 040401 food science 03 medical and health sciences 0404 agricultural biotechnology Contact surfaces Listeria monocytogenes medicine High incidence Food Science |
| Zdroj: | Journal of Food Science and Technology. 55:2808-2812 |
| ISSN: | 0975-8402 0022-1155 |
| Popis: | Listeria monocytogenes was screened from different seafood contact surfaces in five sampling sites of fishing harbour, fish landing centers, seafood processing plants, fish market, and fish curing yards of Tuticorin Coast of India. 115 swab samples were collected and tested for the occurrence of L. monocytogenes by conventional and molecular methods. Overall, 5.22% of swab samples collected were positive for L. monocytogenes. The fishing harbour had high incidence (10.3%) of L. monocytogenes followed by fish landing centers (5.9%), and seafood processing plants (4.1%). Boat deck, fish transport tricycle were the two seafood contact surfaces in fishing harbour, which had the occurrence of L. monocytogenes. The swab samples from fish market and fish curing yards were negative for L. monocytogenes. All the isolated colonies of L. monocytogenes were confirmed by PCR assay targeting virulent hlyA gene. The DNA of all the isolates yielded a product of 174 bp on PCR amplification in comparison with L. monocytogenes Type culture (MTCC 1143). The results clearly indicated the occurrence of L. monocytogenes in seafood contact surfaces along the Tuticorin Coast of India. |
| Databáze: | OpenAIRE |
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