| Autor: |
Ralph J. Rothenberg, Jay I. Goodman |
| Rok vydání: |
1978 |
| Předmět: |
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| Zdroj: |
Journal of Pharmacological Methods. 1:299-309 |
| ISSN: |
0160-5402 |
| DOI: |
10.1016/0160-5402(78)90061-x |
| Popis: |
When hepatic slices prepared from rats subjected to a 70% partial hepatectomy 16 hr earlier are incubated in vitro, they will progress through a wave of DNA synthesis in a manner similar to that seen in vivo. However, slices prepared at 14 hr post-hepatectomy will not initiate DNA synthesis when incubated in vitro. These results were not altered by the addition of either 5% or 20% serum (calf serum or fetal calf serum) to the incubation medium. These results are interpreted as indicating that between 14 hr and 16 hr after partial hepatectomy there exists a critical period during which exposure to a systemic factor(s) commits hepatic cells to enter a round of DNA synthesis. The experimental procedures outlined in this report provide a means to study, in vitro, progression through the S-phase in a normal mammalian cell population. In addition, experiments employing slices prepared at 14 hr post-hepatectomy might aid in discerning factors initiating the G 1 to S-phase conversion. The experimental protocol described is applicable to studies aimed at assessing selected aspects of the effects of drugs and putative toxicants on hepatic function. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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