Preparation and Isolation of the Partially Hydrolyzed Moiety of Fumonisin B1
| Autor: | Klaus R. Koch, Gordon S. Shephard, Pieter G. Thiel, Therese Hutton, Eric W. Sydenham |
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| Rok vydání: | 1995 |
| Předmět: |
Fusarium
Fumonisin B1 Calcium hydroxide Chromatography biology food and beverages General Chemistry Alkaline hydrolysis (body disposal) biology.organism_classification chemistry.chemical_compound Hydrolysis chemistry Yield (chemistry) Fumonisin Organic chemistry Moiety General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 43:2400-2405 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf00057a016 |
| Popis: | The natural occurrence in corn of carcinogenic mycotoxins, the fumonisins, has prompted the development of potential decontamination procedures. Chemical treatment of fumonisin B 1 (FB 1 )-contaminated corn with calcium hydroxide [Ca(OH) 2 ] results in the base hydrolysis of FB 1 (the major naturally occurring fumonisin analogue) to yield its corresponding aminopentol (AP 1 ) and tricarballylic acid (TCA) moieties. Complete hydrolysis proceeds in a sequential reaction involving the removal of one TCA group and the formation of a partially hydrolyzed moiety (PH1), which exists as an equilibrium mixture of the two possible monoesters. PH1 was prepared by the treatment of Fusarium moniliforme culture material with Ca(OH) 2 and subsequently isolated and purified using chromatographic methods. PH 1 was also prepared, using similar methods, from pure FB 1. The identity of the PH 1 moiety was determined by liquid chromatography-electrospray mass spectrometry. |
| Databáze: | OpenAIRE |
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