Phenotypic and molecular detection of biofilm production by P. aeruginosa isolated from different clinical cases
| Autor: | Saadiyah Abed Jaddoa, Mithal K A. Al-Hassani |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International journal of health sciences. :5173-5179 |
| ISSN: | 2550-696X 2550-6978 |
| DOI: | 10.53730/ijhs.v6ns8.13416 |
| Popis: | This research was performed in the aim of detection the ability of Pseudomonas aeruginosa to form a biofilm. So 100 sample were collected from hospitalized and reviewed patients with a different clinical cases including (urinary tract infections, respiratory tract infections, burns, andotitis), both sexes and different ages,at Al-Sadiq hospital in BabilGovernorate, as well as Al‑Diwaniyah Teaching hospital, Burns hospital, Women & Childrenhospital in Al‑Diwaniyah Governorate.Samples were collected from October 2021 until January 2022. 20 isolate of Pseudomonas aeruginosa were obtained. The detection of biofilm formation was performed by using two conventional methods, and all isolates was able to form a biofilms. Thena genetic detection was performed throughthe search for the presenceofalgD, pelF, and pslD genes thatencode for a biofilm production. The results showed the presence of algDgene in a percentage of 65%, while the presence of pelF gene was equal to psld gene, which reached a percentage of 70%, while some isolates contained both genes, others contained pelF gene and lake ofpslD gene or vice versa, the percentage of isolates that contained all three genes was 35%, and the percentage of isolates that contained only one gene was 30%. |
| Databáze: | OpenAIRE |
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