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We studied the acute and sub-acute toxicity of Combretum dolichopetalum leaves in experimental mice and rats respectively using standard techniques. The LD 50 of the methanol extract of Combretum dolichopetalum leaves as carried out in experimental mice was obtained as more than 5000 mg/kg body weight. Administration of graded doses (100, 200, 400 and 800 mg/kg) of the extract for 21 days resulted in increases in body weights, white blood cells (WBC), Neutrophils, red blood cells (RBC), packed cell volume (PCV), haemoglobin (HGB), mean corpuscular volume (MCV) and mean cell haemoglobin (MCH) of the rats; but did not affect (P>0.05) their monocytes, mean cell haemoglobin concentration (MCHC), platelet (PLT) levels. All doses of the extract did not affect (P>0.05) the sodium, potassium, chloride, bicarbonate, urea, creatinine, total and conjugated bilirubin, alanine and aspartate amino transaminase, aspartate amino transaminase, alkaline phosphatase activities; relative liver and kidney weights of the rats, a finding that was corroborated by histology of the liver and the kidney. The extract at 100 mg/kg had no effect on the PCV and HB of the rats. The study suggested the therapeutic potentials of Combretum dolichopetalum as a blood booster. Finally, the study revealed the safety in the usage of Combretum dolichopetalum leaves in Nigerian ethnomedicine. Key words: ethnopharmacology; Combretum dolichopetalum ; toxicology; herbal medicine; nutraceutical; pharmacotherapy AKUTNA IN SUBAKUTNA A TUDIJA TOKSIAŒNOSTI LISTOV RASTLINE Combretum dolichopetalum Povzetek: S standardnimi metodami smo pri poskusnih miAiih in podganah prouAevali akutno in subakutno toksiAnost listov Combretum dolichopetalum . LD 50 metanolnega izvleAka listov Combretum dolichopetalum je bil pri poskusnih miAiih nad 5000  mg/kg telesne teA¾e. Enaindvajsetdnevno dodajanje naraAiAajoAih odmerkov (100, 200, 400 in 800 mg/kg) izvleAka je pri poskusnih podganah povzroAilo poveAanje telesne mase, Aitevila belih krvniAk (WBC), nevtrofilcev, rdeAih krvniAk (RBC), volumna stisnjenih eritrocitov (PCV), hemoglobina (HGB), povpreAnega volumna eritrocitov (MCV) in povpreAno vsebino hemoglobina v eritrocitih (MCH), ni pa vplivalo (p > 0,05) na Aitevilo monocitov, povpreAno koncentracijo hemoglobina v volumnu eritrocitov (MCHC) ter na povpreAno vrednost trombocitov (PLT). Nobeden od odmerkov izvleAka ni vplival na (p > 0,05) vrednosti natrija, kalija, klorida, bikarbonata, seAnine, kreatinina, skupnega bilirubina in vezanega bilirubina, alanina, aspartatne amino transaminaze, aspartatne amino transaminaze, alkalne fosfataze; relativno teA¾o jeter in ledvic podgan, kar je bilo v skladu s histoloAiko preiskavo jeter in ledvic. IzvleAek v odmerku 100 mg/kg ni vplival na PCV in HB podgan. A tudija tako kaA¾e na moA¾nost uporabe rastline Combretum  dolichopetalum za izboljAianje krvne slike. Raziskava je dokazala varnost uporabe listov Combretum dolichopetalum , ki se tradicionalno uporabljajo v Nigeriji v etnomedicini. KljuAne besede: etnofarmakologija; Combretum dolichopetalum ; toksikologija; zeliAiAna zdravila; hranila; farmakoterapija |
