Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Autor:	Carles de Mas, Daniel Calleja, Josep López-Santín, Martina Pasini, Alfred Fernández-Castané
Rok vydání:	2014
Předmět:	Fuculose Environmental Engineering biology Aldolase A Biomedical Engineering lac operon Bioengineering medicine.disease_cause carbohydrates (lipids) chemistry.chemical_compound Biochemistry chemistry biology.protein medicine Extracellular Inducer Escherichia coli Intracellular Biotechnology
Zdroj:	Biochemical Engineering Journal. 91:210-219
ISSN:	1369-703X
DOI:	10.1016/j.bej.2014.08.017
Popis:	An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β- d -tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 Δ glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC–MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::18c7c999054a4bbb8d5b1792680f74ee https://doi.org/10.1016/j.bej.2014.08.017 Zobrazit plný text záznamu Full Text from ScienceDirect