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To quantify vascularity of antral follicles in Bos indicus using Factor VIII immunohistochemistry in order to investigate the relationship between follicular size, health status and vascularity, bovine ovaries were obtained on d 6 of the estrous cycle from mature B. indicus (n=8) after a synchronized estrus with PGF2α analogue. The ovaries were perfused and used for immunofluorescence detection of Factor VIII (a marker of endothelial cells). Follicles were classified by size (small, medium, and large) and by morphology as healthy and atretic follicle. Quantitative analysis was determined by the aid of image analysis. Factor VIII binding was localized exclusively in the thecal layer and stroma of the antral follicles, but was not found in the granulosa layer. For all follicles evaluated, capillary area density (CAD) and capillary number density (CND) were greater (P |
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