DEVELOPMENT OF AN HPLC METHOD WITH UV DETECTION FOR THE PHARMACEUTICAL QUALITY CONTROL OF THE NOVEL MARINE ANTICANCER AGENT KAHALALIDE F
| Autor: | M. Bouma, Auke Bult, Pablo Floriano, E. Stokvis, Bastiaan Nuijen, Hilde Rosing, Manada C, Jos H. Beijnen, J. J. Kettenes-van den Bosch |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Depsipeptide
chemistry.chemical_classification Electrospray Chromatography Clinical Biochemistry Pharmaceutical Science Reversed-phase chromatography Biochemistry High-performance liquid chromatography Cyclic peptide Dosage form Analytical Chemistry chemistry In vivo Quantitative analysis (chemistry) |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 24:3141-3155 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1081/jlc-100107725 |
| Popis: | Kahalalide F is a cyclic depsipeptide derived from the marine mollusc Elysia rufescens, an organism living in the seas near Hawaii. On the basis of its in vitro and in vivo selectivity, kahalalide F is currently developed as a potential anticancer agent against androgen independent prostate tumors. The development and validation of a reversed-phase high performance liquid chromatography (RP-HPLC) method with ultra-violet (UV) detection for the quantification and purity determination of kahalalide F in raw drug substance and pharmaceutical dosage form is described. Linear calibration curves in the range of 0.5–12.5 μg/mL of kahalalide F with correlation coefficients > 0.999 were obtained. Within-run and between-run precisions were ≤ 3.0% and accuracy was within 100.4–103.2%. The assay proved selective, as determined by stress-testing, confirming its stability indicating capacity. Using liquid chromatography-mass spectrometry (LC-MS) analysis, kahalalide G, the hydrolyzed open-chain analog of kahalalide F, ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |