| Autor: |
M. F. Abu Bakar, Azis Saifudin, I.D. Kusumaningrum, Maryati, E. Sutrisna |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Food Research. :755-760 |
| ISSN: |
2550-2166 |
| Popis: |
Cancer is one of the main causes of death globally. Thus, research to develop a new cancer drug is still ongoing. Physallis angulata L. (ceplukan) is one of the Indonesian plants that has been shown to display anti-cancer activity. This research aimed to examine the cytotoxic effect of ethanol extract of P. angulata L., the effect on cell proliferation and the apoptosis-inducing ability against myeloma cells. P. angulata L. plant was extracted using 96% ethanol. The observations of the cytotoxic effect of extract and the proliferation of myeloma cells were done by MTT assay. The effect of the extract on apoptosis was determined by double staining method using ethidium bromide-acridine orange. Markers for apoptosis were measured by immunocytochemistry test. The results showed that the extract of P. angulata exhibited cytotoxic effects on myeloma cells with an IC50 value of 70.92 µg/ml. The extract inhibited the proliferation of myeloma cells and induced apoptosis in myeloma cancer cells by upregulating the expression of p53 and Bax. This result suggested that ethanolic extract of P. angulata L. had the potential to be developed as blood cancer drug. Further study on the isolation of the bioactive compound and in vivo mechanism is in progress. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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