Development of an aqueous temperature-indicating technique and its application to clinical laboratory instrumentation

Autor:	L Bowie, Jeffrey T. Bolin, F Esters, N Gochman
Rok vydání:	1976
Předmět:	Aqueous solution Chromatography Instrumentation Biochemistry (medical) Clinical Biochemistry Analytical chemistry Cresol Red Temperature measurement Absorbance chemistry.chemical_compound chemistry Sodium hydroxide Calibration Hydroxymethyl
Zdroj:	Clinical Chemistry. 22:449-455
ISSN:	1530-8561 0009-9147
Popis:	We describe a new technique for monitoring intra-cuvette temperatures in temperature-controlled spectrophotometric cells by use of two solutions, one for which absorbance is highly sensitive to temperature and one for which absorbance is insensitive to temperature. The first solution, cresol red in tris(hydroxymethyl)aminomethane buffer, is used to construct an absorbance-vs.-temperature calibration curvae. The second solution, cresol red in sodium hydroxide, is used for optical calibration of the spectrophotometric system to be tested. The combination of these two procedures allows accurate estimation of temperature. We show how this technique is applied to temperature measurement in several types of laboratory instrumentation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::18b11f5e6f59ace56c3209c44f789e7d https://doi.org/10.1093/clinchem/22.4.449 Zobrazit plný text záznamu