Dichlorodiphenylchloroethylene Elevates Cytosolic Calcium Concentrations and Oscillations in Primary Cultures of Human Granulosa-Lutein Cells1
| Autor: | Derek K. Lobb, T. K. Kwan, Warren G. Foster, Edward V. Younglai, C.-Y. Kwan |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
endocrine system
medicine.medical_specialty food and beverages chemistry.chemical_element Cell Biology General Medicine Calcium Biology environment and public health Follicular fluid enzymes and coenzymes (carbohydrates) Follicle-stimulating hormone Cytosol EGTA chemistry.chemical_compound Endocrinology medicine.anatomical_structure Reproductive Medicine chemistry Internal medicine embryonic structures medicine Ovarian follicle Granulosa Lutein Cell Homeostasis |
| Zdroj: | Biology of Reproduction. 70:1693-1700 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.103.026187 |
| Popis: | Dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a me- tabolite of DDT (1,1-dichlorodiphenyltrichloroethane), is a per- sistent hormonally active environmental toxicant that has been found in human serum and follicular fluid. The objective of this study was to determine whether DDE can alter free calcium ion concentrations in the cytosol ((Ca21)cyt) of human granulosa cells. Changes in (Ca21)cyt in single cells loaded with Fura-2 were studied using a dynamic digital Ca21 imaging system. At a con- centration of 100 ng/ml, DDE stimulated small elevations of (Ca21)cyt accompanied by Ca21 oscillations. At 1 mg DDE/ml, there was a biphasic Ca21 response with marked elevations of (Ca21)cyt over time. In Ca21-free medium, cells showed an initial small elevation of (Ca21)cyt, which was magnified after addition of Ca21 to the medium. Washing the cells after DDE treatment failed to remove the elevated (Ca21)cyt and oscillations, both of which were eliminated by addition of EGTA. ATP also induced (Ca21)cyt elevations and oscillations, and these effects were po- tentiated when DDE was added. FSH induced transient (Ca21)cyt elevations, whereas hCG caused a prolonged elevation and marked oscillations in (Ca21)cyt. These results suggest that DDE at concentrations normally found in human tissues induces el- evations in (Ca21)cyt in granulosa-lutein cells. Our data therefore highlight a novel mechanism through which DDE can alter en- docrine homeostasis and possibly act as an endocrine toxicant. calcium, estradiol, follicle-stimulating hormone, granulosa cells, human chorionic gonadotropin |
| Databáze: | OpenAIRE |
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