Lutein facilitates physiological revascularization in a mouse model of retinopathy of prematurity
Autor: | Steven S. Meng, Amy C. Y. Lo, Zhongjie Fu, Samuel B. Burnim, Lois E.H. Smith |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Lutein Pathology medicine.medical_specialty Ischemia Neovascularization 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine business.industry Retinal Retinopathy of prematurity medicine.disease eye diseases Surgery Ophthalmology 030104 developmental biology medicine.anatomical_structure chemistry Gliosis sense organs medicine.symptom business 030217 neurology & neurosurgery Blood vessel Retinopathy |
Zdroj: | Clinical & Experimental Ophthalmology. 45:529-538 |
ISSN: | 1442-6404 |
Popis: | Background Retinopathy of prematurity is one of the leading causes of childhood blindness worldwide, with vessel growth cessation and vessel loss in phase I followed by neovascularization in phase II. Ischemia contributes to its pathogenesis and lutein protects against ischemia-induced retinal damages. We aimed to investigate the effects of lutein on a murine model of oxygen-induced retinopathy. Methods Mouse pups were exposed to 75% oxygen for five days and returned to room air for another five days. Vascular obliteration, neovascularization and blood vessel leakage were examined. Immunohistochemistry for glial cells and microglia were performed. Results Compared with vehicle controls, mouse pups receiving lutein treatment displayed smaller central vaso-obliterated area and reduced blood vessel leakage. No significant difference in neovascular area was found between lutein and vehicle controls. Lutein promoted endothelial tip cell formation and maintained the astrocytic template in the avascular area in oxygen-induced retinopathy. No significant changes in Muller cell gliosis and microglial activation in the central avascular area were found in lutein-treated pups. Conclusions Our observations indicated that lutein significantly promoted normal retinal vascular regrowth in the central avascular area, possibly through promoting endothelial tip cell formation and preserving astrocytic template. Our results indicated that lutein might be considered as a supplement for the treatment of proliferative retinopathy of prematurity due to its role in facilitating the revascularization of normal vasculature. |
Databáze: | OpenAIRE |
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