Virus-Induced Gene Silencing in Olive Tree (Oleaceae)
| Autor: | Stamatis Rigas, Polydefkis Hatzopoulos, Elisavet Angeli, Konstantinos Koudounas, Margarita Thomopoulou, Dikran Tsitsekian |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Period (gene) Agrobacterium tumefaciens Computational biology Biology biology.organism_classification 01 natural sciences 03 medical and health sciences Tree (data structure) 030104 developmental biology Tobacco rattle virus Oleaceae Gene silencing Gene Functional genomics 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071607503 |
| Popis: | Research on gene functions in non-model tree species is hampered by a number of difficulties such as time-consuming genetic transformation protocols and extended period for the production of healthy transformed offspring, among others. Virus-induced gene silencing (VIGS) is an alternative approach to transiently knock out an endogenous gene of interest (GOI) by the introduction of viral sequences encompassing a fragment of the GOI and to exploit the posttranscriptional gene silencing (PTGS) mechanism of the plant, thus triggering silencing of the GOI. Here we describe the successful application of Tobacco rattle virus (TRV)-mediated VIGS through agroinoculation of olive plantlets. This methodology is expected to serve as a fast tracking and powerful tool enabling researchers from diversified fields to perform functional genomic analyses in the olive tree. |
| Databáze: | OpenAIRE |
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