Conditional immortalization of rodent fibroblasts using a temperature sensitive mutant fragment of polyoma virus large T antigen

Autor:	J. Bishop, N. Krauzewicz, Y. Hamashima, M. Towey, B. E. Griffin
Rok vydání:	1995
Předmět:	Rodent biology Oncogene Cell Biology Temperature-sensitive mutant Virology Molecular biology Virus medicine.anatomical_structure Cell culture biology.animal medicine Primary cell Fibroblast Function (biology)
Zdroj:	Methods in Cell Science. 17:111-116
ISSN:	1573-0603 1381-5741
DOI:	10.1007/bf00986659
Popis:	We describe a method for prolonging the life span of primary cells using an N-terminal fragment of a temperature sensitive mutant of the polyoma virus oncogene, its large T antigen (LT). This allows long term characterization studies to be carried out on cells which would otherwise have senesced after a few passages in culture. Further, repeated preparation of cells from tissue sources can be avoided. Moreover, the cells can also be reverted to a 'mortal' state, if desired, by incubating at the non-permissive temperature for the LT function.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1885d99fcd4cfb95a111e184418486a1 https://doi.org/10.1007/bf00986659 Zobrazit plný text záznamu Full text from SpringerLink