| Autor: |
Longzhu Cui, Kazuyuki Mima, Yuuichi Ishida, Katsuo Sato, Hirofumi Shimomura, Yoshikazu Hirai, Einori Sugiyama, Akira Taneno, Eishi Sakai |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Food Safety. 7:58-59 |
| ISSN: |
2187-8404 |
| DOI: |
10.14252/foodsafetyfscj.2018020 |
| Popis: |
Salmonella enterica subsp. enterica serovar Enteritidis (SE) is one of the major causes of food poisoning. Much effort has been made to develop a vaccine for the prevention of SE colonization and infection in poultry. However, the effect of inactivated whole-cell SE vaccines on the bacterial attachment has not been clarified. This study investigated the immune responses to a killed whole-cell SE vaccine in chickens and the effect of vaccination on the bacterial attachment of SE to cultured Vero cells. A 1 ml dose of 108-109 CFU viable SE bacterial cells was orally administered to chickens at 4 weeks or 10 months post vaccination. The number (CFU) of SE in 1 g of cecal droppings was counted on day 6 after administration. The SE CFUs were significantly lower (p < 0.05) in the vaccinated chickens, not only at 4 weeks but also at 10 months after vaccination, than in the unvaccinated control chickens. Anti-SE IgG and anti-SE IgA were detected using enzyme-linked immunosorbent assay (ELISA) in serum and intestinal and oviduct fluid samples from vaccinated chickens. Adhesion of heat-killed SE cells to Vero cells was reduced by pre-treatment of the bacteria by the vaccinated chicken-derived intestinal fluid, indicating the potential of the vaccine-induced antibody to prevent SE adhesion to epithelial cell surfaces. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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