A Story of Chelatase Evolution
| Autor: | Amanda A. Brindley, Martin J. Warren, Helen K. Leech, Evelyne Raux, Heidi L. Schubert |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Genetics
ved/biology ved/biology.organism_classification_rank.species Cobaltochelatase Sequence alignment Cell Biology Biology medicine.disease_cause Biochemistry Conserved sequence Sirohydrochlorin cobaltochelatase Protein structure medicine Methanosarcina barkeri Molecular Biology Escherichia coli Peptide sequence |
| Zdroj: | Journal of Biological Chemistry. 278:22388-22395 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m302468200 |
| Popis: | The cobaltochelatase required for the synthesis of vitamin B12 (cobalamin) in the archaeal kingdom has been identified as CbiX through similarity searching with the CbiX from Bacillus megaterium. However, the CbiX proteins in the archaea are much shorter than the CbiX proteins found in eubacteria, typically containing less than half the number of amino acids in their primary structure. For this reason the shorter CbiX proteins have been termed CbiXS and the longer versions CbiXL. The CbiXS proteins from Methanosarcina barkeri and Methanobacter thermoautotrophicum were overproduced in Escherichia coli as recombinant proteins and characterized. Through complementation studies of a defined chelatase-deficient strain of E. coli and by direct in vitro assays the function of CbiXS as a sirohydrochlorin cobaltochelatase has been demonstrated. On the basis of sequence alignments and conserved active site residues we suggest that CbiXS may represent a primordial chelatase, giving rise to larger chelatases such as CbiXL, SirB, CbiK, and HemH through gene duplication and subsequent variation and selection. A classification scheme for chelatases is proposed. |
| Databáze: | OpenAIRE |
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