The Comparative Susceptibility of the Diamondback Moth Plutella xylostella and Some Other Major Lepidopteran Pests of Brassica Crops to a Range of Baculoviruses

Autor: Norman E. Crook, Hussan Bin Abdul Kadir, Christopher C. Payne, Doreen Winstanley, John S. Fenlon
Rok vydání: 1999
Předmět:
Zdroj: Biocontrol Science and Technology. 9:421-433
ISSN: 1360-0478
0958-3157
Popis: The susceptibility of larvae of the Diamondback moth (DBM), Plutella xylostella to infection by three baculoviruses was evaluated in the laboratory using a microdroplet feeding assay. The viruses tested were a granulovirus (GV), originally isolated in Taiwan from P. xylostella larvae (Px GV-Taiwan); the nucleopolyhedrovirus (NPV) from Galleria mellonella (Gm NPV), and the NPV from Autographa californica (Ac NPV). Neonate P. xylostella larvae were susceptible to infection by all three viruses. In an extensive series of bioassays carried out over a 21-month period, LD 50S for neonate DBM larvae ranged from 1.0-8.9 viral occlusion bodies (OB) for Px GV-Taiwan, and 9.5-30.2 OB for Gm NPV and Ac NPV. LT 50S for the three viruses ranged from 3.8-6.0 days at 27 C, with Gm NPV having a significantly shorter LT 50 than the other two viruses. Second and third instar larvae of P. xylostella were significantly less susceptible to infection by Px GV-Taiwan (LD 50 s ranging from 18-57 OB/larva) than were neonate larvae...
Databáze: OpenAIRE