Abstract 372: CD16 Monocytes Stimulate Arteriogenesis to Salvage the Ischemic Limb
| Autor: | Ashish S Patel, Francesca E Ludwinski, Puja Kapoor, Paul R Jones, Alexander Kerr, Tamer El-Sayed, Prakash Saha, Oliver T Lyons, Alberto Smith, Bijan Modarai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Circulation Research. 121 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/res.121.suppl_1.372 |
| Popis: | Introduction: Cell therapy using unselected mononuclear cell populations has had modest benefits in patients with critical limb ischemia (CLI). We hypothesized that tissue-remodeling monocytes, identified by their expression of CD16 (CD16+ Mo), may be a novel cellular therapy for CLI. Methods and Results: Flow cytometry showed that the proportion of circulating CD16+ Mo was greater in CLI patients (n=25) compared with matched controls (n=15, P6 cells injected into the adductor muscles of nude athymic mice following femoral artery excision. More ischemic hindlimbs were salvaged when treated with CD16+ compared with CD16- Mo (83% [10/12] vs 17% [2/12] limbs, P Conclusion: Circulating CD16+ Mo from CLI patients have increased expression of adhesion markers, are preferentially retained within ischemic muscle and promote robust arteriogenesis and limb salvage in experimental HLI. This monocyte subset may be an effective cellular therapy for CLI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|