| Popis: |
Background Integrated data from culturomics and functional omics may depict holistic understanding on gut microbiome eubiosis or dysbiosis, and microbial isolates can become a source of differential enzymes and useful bioactive compounds. Culturing methods developed during last decade swift increases the importance of gut microbial isolates, focusing on media, modifications and conditions that propitiate cultured taxa that previously were considered fastidious or unculturable. In this context and focusing on gut microbiota dysbiosis triggered by obesogens and microbiota disrupting chemicals (MDC), we have conducted a directed-culturing and bioinformatics combined approach, adding bisphenol A (BPA) and specific treatments to find resistant spore-forming bacteria, to obtain isolated strains for further explore their molecular BPA metabolizing or neutralizing capacities. Results Overall microbiota culturing media and conditions have been retrieved and organized according to main gut taxa isolated during last decade. Furthermore, a catalogue of BPA directed-cultured microorganisms has been obtained from 46 fecal samples from two populations, children with obesity and normo-weight. A total of 235 BPA tolerating and potentially BPA biodegrading microorganisms were mainly grouped to strictly anaerobic sporuled/non-sporuled, anaerobic facultative sporuled/non-sporuled. Firmicutes, Enterobacteria and Actinobacteria species showed the major representation in both groups. However, differential BPA tolerant microbiota composition from the populations was detected. Bioinformatics analysis disclosed and predicted the variability of harboring genes encoding specific enzyme for BPA biodegradation pathways that corroborated from directed-culturing microbiota consortia obtained. Conclusions Strains from Staphylococcus , Bacillus and Enterococcus genera represented the majority of the successfully cultured bacteria in both population specimens. From them, the bioinformatics prediction assigned to Bacillus spp. the higher potential for BPA biodegradation. Therefore, extensive directed-culturomics approaches could be designed for different MDC with common biodegradation pathways, such as parabens, phthalates, and benzophenones. |
