| Autor: | Ziya Gunata, Marie-Jose Vallier |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Aspergillus Molecular mass biology Aspergillus niger Size-exclusion chromatography Bioengineering General Medicine Cellobiose biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Enzyme chemistry Aspergillus oryzae Biochemistry Extracellular Biotechnology |
| Zdroj: | Biotechnology Letters. 21:219-223 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1005407710806 |
| Popis: | Quercetin was the best inducer for the production of a highly glucose-tolerant, extracellular β-glucosidase in Aspergillus niger and Aspergillus oryzae. The enzyme was separated from the major and common β-glucosidase by gel filtration and that from Aspergillus oryzae further purified by ion-exchange chromatography. It was highly resistant to glucose inhibition (Ki= 953 mM), had a pI of 4.2, optimum pH of 4.5–6.0 and a molecular mass of 30 kDa according to gel filtration. The enzyme was active against cellobiose and alkyl glucosides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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