Rice CaM-binding transcription factor (OsCBT) mediates defense signaling via transcriptional reprogramming
| Autor: | Jung-Sung Chung, Dongwon Baek, Man Soo Choi, Wook-Hun Jung, Ki-Hong Jung, Cheol Woo Choi, Anil Kumar Nalini Chandran, Jong-Il Chung, Seon-In Yeom, Hyun Min Cho, Hyun Jin Chun, Sung Cheol Koo, Su Hyeon Lee, Sang In Shim, Min Chul Kim, Byung Jun Jin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Microarray Mutant Plant Science Biotic stress Biology 01 natural sciences Cell biology 03 medical and health sciences 030104 developmental biology Downregulation and upregulation Gene expression Gene Transcription factor 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Reports. 14:309-321 |
| ISSN: | 1863-5474 1863-5466 |
| DOI: | 10.1007/s11816-020-00603-y |
| Popis: | The mutant allele of rice calmodulin-binding transcription activator OsCBT, oscbt-1, exhibits broad-spectrum resistance against rice pathogens. Previously, we reported that the strong resistance of the oscbt-1 mutant to pathogens was conferred by a constitutive upregulation of defense-related genes even under pathogen-free conditions. We also found strong induction of the hypersensitive response as a reaction to pathogen invasion. The results suggest that OsCBT acts as a negative regulator of basal resistance to pathogen attack. To identify the transcriptional network regulated by OsCBT, we compared global gene expression profiles between wild-type (WT) and oscbt-1 rice plants grown under pathogen-free conditions. The results of a 3′ tiling microarray revealed that in oscbt-1 plants, 81 genes are upregulated and 200 genes are downregulated when compared with the WT. A gene ontology analysis showed that differentially regulated genes in oscbt-1 were very closely associated with “death” GO term in a biological process category, and “catalytic activity” and “binding” GO terms in a molecular function category. A MapMan analysis indicated that the functions of these genes were associated with plant responses to biotic stress. Moreover, the results from quantitative real-time PCR in the oscbt-1 mutant showed a significant effect on the gene expression patterns of the fungal pathogen response. Our results suggested that the OsCBT regulates a rice defense response by modulating the expressions of various defense-related genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink