| Autor: |
V. Nádudvari-Márkus, L. Vámos-Vigyázó, A. Párkány-Gyárfás |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography A. 241:229-235 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(00)82411-5 |
| Popis: |
The amylase isoenzyme compositions of an early- and a mid-season-ripening variety of Hungarian wheat were investigated, during ripening and storage, by polyacrylamide disc gel electrophoresis. Of the two electrophoretic systems studied, the alkaline one (pH 7.9) gave a higher resolution and was used throughout the study. Amylase fractions detected on starch and β-limit-dextrin substrates, were studied in parallel with quanititative amylase activity determinations. Isoenzymes acting on β-limit dextrin (α-amylase) were detectable only during the first half of the ripening period. The number of the fractions detected on starch decreased during the first half of the ripening period and remained practically unchanged during the second half. Amylase activity varied in a similar way. The number of amylase isoenzymes increased during storage, while amylase activity showed but minor fluctuations. The increase in the number of isoenzymes during storage might be due to the dissociation of molecular aggregates of the enzyme. In the samples taken throughout ripening and storage these remained in the large-pore sample gel. The isoenzyme pattern of wheat seems to be primarily dependent on the stage of ripening and only to a lesser degree on the variety. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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