In vivo administration of a thiol protease inhibitor, E-64-C, to hereditary dystrophic chicken
| Autor: | Masahiro Ohzeki, Masaharu Tamai, Kazunori Hanada, Shoichi Ishiura, Yasuo Tarumoto, Messrs. Masaaki Kimura, Kazutomo Imahori, Hideo Sugita, Ikuya Nonaka |
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| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Physiology E-64 Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Biochemistry chemistry In vivo Flip Necrotic fibers Physiology (medical) Internal medicine Neutral protease medicine High doses Neurology (clinical) Thiol protease inhibitor Pectoralis Muscle |
| Zdroj: | Muscle & Nerve. 5:738-744 |
| ISSN: | 1097-4598 0148-639X |
| DOI: | 10.1002/mus.880050914 |
| Popis: | Line 413 hereditary muscular dystrophic chickens were given twice daily subcutaneous injections of 150 mg or 300 mg/kg body weight of E-64-c, a potent thiol protease inhibitor, from 10 days ex ovo to 89 days of age. The administration of high doses of E-64-c did not influence the growth curve, the course of the “flip number” the chronological changes of serum CK, PK, and LDH, and the number of the necrotic fibers and also the histogram of the fiber diameters. The activity of Ca2+-activated neutral protease (CANP) in superficial pectoralis muscle was decreased by E-64-c in female chickens, but still remained higher than normal. In males, however, the CANP activity was not decreased at all. The male dystrophic chickens showed heavier body weights, lower flip number, and higher serum enzymes during the course of the experiment and higher CANP activities in superficial pectoralis muscles than females. |
| Databáze: | OpenAIRE |
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