| Autor: |
Radko Rajmon, Markéta Sedmíková, Jaroslav Petr, E. Chmelíková, Dana Švestková, J. Rozinek, Michal Jeseta, F. Jílek |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Molecular Reproduction and Development. 71:115-122 |
| ISSN: |
1040-452X |
| DOI: |
10.1002/mrd.20248 |
| Popis: |
Nitric oxide (NO) plays an impor- tant role in intracellular signaling, but its role during the activation of mammalian oocytes is little understood. In our study, in vitro matured pig oocytes were cultur- ed with NO-donors—S-nitroso-N-acetylpenicillamine (SNAP) or sodium nitropruside (SNP). These treat- ments were able to induce parthenogenetic activation of pig oocytes matured in vitro. The specificity of this effect was confirmed by the activation of oocytes by exogenous endothelial nitric oxide synthase (eNOS) microinjected in the oocyte with its activator calmo- dulin. Relatively long exposure (10 hr) is needed for activation of pig oocytes with 2.0 mM SNAP. An active NOS is necessary for the NO-dependent activation of pig oocytes because NOS inhibitors L-NMMA or L- NAME are able to inhibit activation of oocytes with NO- donor SNAP. On the basis of our data, we conclude that the NO-dependent activating stimulus seems inadequate because it did not induce the exocytosis of cortical granules. Also, the cleavage of parthenoge- netic embryos was very low, and embryos did not develop beyond the stage of eight blastomeres. Mol. Reprod. Dev. 71: 115-122, 2005. 2005 Wiley-Liss, Inc. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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