C. albicans activates cyclooxygenase but not its product prostaglandin E2 in HPV 16-stabilized cells
| Autor: | Nadia Kerstin, Santosh Nigam, Sipra Saha, Maria-Patapia Zafiriou, Per-Johan Jakobsson, Rupal Deva, Marco Sczepanski, Yuichi Inoue |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
biology
business.industry medicine.medical_treatment Obstetrics and Gynecology Cell cycle biology.organism_classification medicine.disease_cause Corpus albicans Multiplicity of infection Reproductive Medicine Immunology medicine Genital neoplasm Prostaglandin E2 Candida albicans business Carcinogenesis medicine.drug Prostaglandin E |
| Zdroj: | European Journal of Obstetrics & Gynecology and Reproductive Biology. 152:205-209 |
| ISSN: | 0301-2115 |
| DOI: | 10.1016/j.ejogrb.2010.06.006 |
| Popis: | Objective The selective induction of cyclooxygenase-2 (COX-2) in human cells by Candida albicans was the first report of its role in infectious disease. This led us to question whether recurrent vulvovaginal candidosis in the cancer patient is involved in the formation of malignant tumors of the genital tract. Our speculation coincided with the patients’ assessments in our hospital, where few cancer patients had a prior history of Candida infection. We wanted to study the contribution of C. albicans to gynecological cancers. Study design In the present study, we used the developed vaginal epithelial cells system, having an insertion of HPV 16 viral sequence, as a model system (VK2/E6E7) to investigate the effect of Candida infection on prostaglandin E2 synthesis, which is known to be associated with cancers. We infected VK2/E6E7 cells with wild-type C. albicans and determined its effect on COX-2 and prostaglandin E 2 synthesis, and its alteration in dependence on p53, and we analyzed the ubiquitin–proteasome degradation pathways and the involvement of 14-3-3 protein, which is involved in the modulation of the cell cycle. Results Our work using the cellular model indicates that recurrent Candida infection of the genital tract in patients carrying HPV 16 viral infection blocks the proliferation of host cells, PGE2 synthase expression and thus PGE2 production. Conclusion We found that Candida infection contributes only to cell cycle arrest and does not by itself contribute actively to the development of cancer, although it is associated with patients diagnosed as having cancer of the genital tract induced by HPV 16 virus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect