Endophytic bacterial biocontrol agents degrade a putative toxin of Alternaria macrospora responsible for the severity of cotton leaf blight
Autor: | Manjunath Hubballi, G. Rajesha, S. Nakkeeran, P. Adhipathi, A. Chandrasekar, T. Indumathi |
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Rok vydání: | 2021 |
Předmět: | |
Zdroj: | Journal of Plant Pathology. 103:1283-1293 |
ISSN: | 2239-7264 1125-4653 |
DOI: | 10.1007/s42161-021-00925-y |
Popis: | The pathogen associated with leaf blight of cotton was isolated and identified as Alternaria macrospora through cultural, morphological characteristics and sequencing of the ITS region. Further, the purified pathogen produced characteristic symptoms of leaf blight disease of cotton on seedlings 10 days after artificial inoculation. The role of putative toxins in leaf blight disease development was studied. Diethyl ether was the most effective solvent, and a culture filtrate using such solvent produced the largest necrotic lesion on cotton and induced maximum electrolytic leakage. It was confirmed that an incubation period of 21 days in Richards broth was optimal to harvest the mycelia to obtain the maximum amount of putative toxin as shown by the largest value of electrolytic leakage in cotton seedlings measured in this experiment. The biochemical analysis of the putative toxin proved its composition included more carbohydrate than protein. Among six endophytic bacteria, B. amyloliquefaciens EBs2 could effectively degrade the putative toxin. Thus, the study concluded that Bacillus isolates could be used for the degradation of toxins produced by Alternaria in cotton. |
Databáze: | OpenAIRE |
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