Evaluation of p-(13C,15N-Cyano)phenylalanine as an Extended Time Scale 2D IR Probe of Proteins
| Autor: | Silas P. Cook, Sashary Ramos, Amanda L. Le Sueur, Jonathan D. Ellefsen, Megan C. Thielges |
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| Rok vydání: | 2017 |
| Předmět: |
Infrared
Chemistry Analytical chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Spectral line 0104 chemical sciences Analytical Chemistry Isotopic labeling Absorbance Temporal resolution 0210 nano-technology Spectroscopy Absorption (electromagnetic radiation) Plastocyanin |
| Zdroj: | Analytical Chemistry. 89:5254-5260 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b04650 |
| Popis: | Two-dimensional infrared (2D IR) spectroscopy provides a powerful approach for the direct study of molecular dynamics with high spatial and temporal resolution. Its application for investigating specific locations in proteins requires the incorporation of IR probe groups with spectrally isolated absorptions to avoid the congestion inherent to protein spectra. This has motivated extensive efforts toward the development of new IR probes, but there remains a need for those that can extend the experimental time range, which is limited by their vibrational lifetimes. Toward this goal, isotopically labeled p-(13C15N-cyano)phenylalanine was synthesized, site-selectively incorporated into the protein plastocyanin, and evaluated for its potential as a 2D IR probe. The isotopic labeling increases the vibrational lifetime about 2-fold, which results in larger signals at longer time scales. However, isotopic labeling simultaneously shifts the absorption to a spectral region with greater water absorbance, which result... |
| Databáze: | OpenAIRE |
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