Mitochondrial DNA deletions in the peripheral blood of workers at the Mayak PA who were exposed to long-term combined effects of external γ- and internal α-radiation

Autor: V. N. Antipova, Kirillova En, L. V. Malakhova, Sokolova Sn, M. G. Lomaeva, M L Zakharova, V. G. Bezlepkin
Rok vydání: 2016
Předmět:
Zdroj: Biophysics. 61:1026-1032
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s0006350916060142
Popis: The levels of large deletions in the mitochondrial DNA of workers at the Mayak Production Association (Mayak PA) who were exposed to external and combined occupational (external γ- and internal α-rays) radiation during the course of their duties were investigated. Peripheral blood-derived DNA samples were provided by the Radiobiological Human Tissue Repository of the Southern Urals Biophysics Institute (Russia). The samples were analyzed using long-extension PCR. The number of large-scale deletions in the mitochondrial DNA of workers who, in addition to external γ-radiation, were exposed to extra doses of irradiation due to incorporated 239Pu with a Pu body burden of 0.77–4.32 kBq, was 2.5-times lower compared to that of individuals who received only external γ-radiation. No significant gender-associated effects on the number of mitochondrial DNA deletions were detected among age-matched individuals.
Databáze: OpenAIRE
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