| Autor: |
Кuksova, А.N., Sitko, V.L., Abdurashitov, M.A., Akishev, A.G., Netesova, N.A., Vihlyanov, I.V., Nikitin, M.K., Karpov, A.B., Degtyarev, S.Kh. |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.26213/se.2019.91.42836 |
| Popis: |
Methylation of ACGC site in position Chr11: 65647266 (according to GRCh38 PrimaryAssembly) in DNA samples from blood cells of healthy donors and early stage renal cancer patients was studied with GLAD-PCR assay. The work includes a) preparation of light fraction of the blood cells, b) isolation of DNA from a light fraction of the blood cells and determination of DNA concentration by real-time PCR, c) determination of A(5mC)GC site (Chr11: 65647266) concentration in DNA preparations by GLAD-PCR assay and d) calculation of percent of DNA molecules with A(5mC)GC site in position Chr11: 65647266. It was shown that a methylation of ACGC site (Chr11: 65647266) in DNA preparations from a light fraction of the blood cells of the early stage renal cancer patients is varied from 96 to 126 . At the same time this site methylation in DNA preparations from a light fraction of the blood cells of conditionally healthy donors lays in a range 72 85 . Thus, GLAD-PCR assay of A(5mC)GC site (Chr11: 65647266) in DNA preparations from a light fraction of the blood cells allows to exclude early stage renal cancer. GLAD PCR assay doesnt require special and expensive equipment. The study can be carried out in a standard PCR laboratory when a blood analysis is taken place. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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