Quantitation of brain metabolites by HRMAS-NMR spectroscopy in rats exposed to sublethal irradiation
Autor: | F. Fauvelle, C. Cretallaz, C. Amourette, P. Martigne, M. Diserbo, S. Follot |
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Rok vydání: | 2008 |
Předmět: |
medicine.medical_specialty
Renewable Energy Sustainability and the Environment Chemistry Health Toxicology and Mutagenesis Neurogenesis Central nervous system Public Health Environmental and Occupational Health Hippocampus Striatum Total body irradiation Pathophysiology Endocrinology medicine.anatomical_structure Nuclear magnetic resonance Nuclear Energy and Engineering Hypothalamus Internal medicine Cortex (anatomy) medicine Safety Risk Reliability and Quality Waste Management and Disposal |
Zdroj: | Radioprotection. 43 |
ISSN: | 1769-700X 0033-8451 |
DOI: | 10.1051/radiopro:2008647 |
Popis: | Puropose : In the event of an acute total-body irradiation, whatever it is therapeutic or accidental, the physiopathology explaining the long-term neurological effects is unknown. We have developed a model of adult rats for which frequent behavioral assays were performed before and after a non-lethal whole-body ionizing radiation (60Co, 4,5 Gy). Learning and memory processing is an aspect of cognition involving mainly the hippocampus. We used high-resolution magic angle spinning (HRMAS) 1H NMR spectroscopy to characterize the biochemistry of four specific brain regions. The biological data for each animal will be compared to their behavioral performances, in order to underline any possible correlations. The best understanding of the physiopathological process in the Central Nervous System (CNS) will allow determining some prevention means or some enhancements for the radio-induced neurological late effects. Experimental procedures: Twenty male Wistar rats were experimented for each sample period, among which ten were gamma radiated (4.5 Gy). The cerebral structures (cortex, striata, anterior and posterior hippocampus, hypothalamus) were removed at three times: 48 hours, 8 days and 30 days after radiation. The HRMAS 1H NMR experiments were performed on a Bruker DRX Avance spectrometer at 9.4 T. Samples were spun at 4 kHz and the temperature maintained at 4°C. A spin-echo sequence with a 30ms total echo time was used. Eighteen metabolites were included in the basis. They were quantitated using the quest procedure of JMRUI software, and statistically analyzed. Moreover, another group of animals was radiated and tested in the same conditions. Then a immuno-histological study of apoptosis and neurogenesis events in the CNS was made at the same removal times. Results: NMR HRMAS results present significant differences (p |
Databáze: | OpenAIRE |
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