A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells
| Autor: | Scott A Lesley, Scott Martin Glaser, Paul Anderson, Sarah Rue, Jessica J. Miller, Michelle R Gaylord |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0303 health sciences Drug discovery Chemistry Chinese hamster ovary cell HEK 293 cells Protein engineering 01 natural sciences Embryonic stem cell Cell biology 03 medical and health sciences Cell culture 010608 biotechnology Protein purification Protein biosynthesis 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493996230 |
| DOI: | 10.1007/978-1-4939-9624-7_5 |
| Popis: | Recombinant protein expression and purification is an essential component of biomedical research and drug discovery. Advances in automation and laboratory robotics have enabled the development of highly parallel and rapid processes for cell culture and protein expression, purification, and analysis. Human embryonic kidney (HEK) cells and Chinese hamster ovary (CHO) cells have emerged as the standard host cell workhorses for producing recombinant secreted mammalian proteins by using both transient and stable production strategies. In this chapter we describe a fully automated custom platform, Protein Expression and Purification Platform (PEPP), used for transient protein production from HEK cells and stable protein production from CHO cells. Central to PEPP operation is a suite of custom robotic and instrumentation platforms designed and built at GNF, custom cell culture ware, and custom scheduling software referred to as Runtime. The PEPP platform enables cost-effective, facile, consistent production of proteins at quantities and quality useful for early stage drug discovery tasks such as screening, bioassays, protein engineering, and analytics. |
| Databáze: | OpenAIRE |
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