Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins

Autor:	Won Do Heo, Byung Ouk Park, Byung-Ha Oh, Hansol Lee, Daseuli Yu, YoungJu Jo, Jongryul Hong, Hyunjin Jung
Rok vydání:	2019
Předmět:	0303 health sciences biology Chemistry Endogeny macromolecular substances Cell Biology Optogenetics Biochemistry Antibody fragments Cell biology 03 medical and health sciences biology.protein Antibody Receptor Molecular Biology Gene Gelsolin Intracellular 030304 developmental biology Biotechnology
Zdroj:	Nature Methods. 16:1095-1100
ISSN:	1548-7105 1548-7091
Popis:	Intracellular antibodies have become powerful tools for imaging, modulating and neutralizing endogenous target proteins. Here, we describe an optogenetically activated intracellular antibody (optobody) consisting of split antibody fragments and blue-light inducible heterodimerization domains. We expanded this optobody platform by generating several optobodies from previously developed intracellular antibodies, and demonstrated that photoactivation of gelsolin and β2-adrenergic receptor (β2AR) optobodies suppressed endogenous gelsolin activity and β2AR signaling, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::17c04d2437bb63117d06ba7b8b318180 https://doi.org/10.1038/s41592-019-0592-7 Zobrazit plný text záznamu Full text from SpringerLink