A Real-Time Multiplex PCR Assay for Detection of Elizabethkingia Species and Differentiation between Elizabethkingia anophelis and E. meningoseptica
| Autor: | Melissa Bell, Ben W. Humrighouse, Aubree J. Kelly, Christopher A. Gulvik, Ainsley C. Nicholson, John R. McQuiston, Sandor E. Karpathy, Anne M. Whitney, Melissa L. Ivey |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Comparative genomics food.ingredient Elizabethkingia Biology medicine.disease_cause Molecular diagnostics biology.organism_classification law.invention Microbiology food law Multiplex polymerase chain reaction Elizabethkingia anophelis medicine Multiplex Elizabethkingia meningoseptica Polymerase chain reaction |
| Zdroj: | Journal of Clinical Microbiology. 57 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01619-18 |
| Popis: | Nosocomial infections of Elizabethkingia species can have fatal outcomes if not identified and treated properly. The current diagnostic tools available require culture and isolation, which can extend the reporting time and delay treatment. Using comparative genomics, we developed an efficient multiplex real-time PCR for the simultaneous detection of all known species of Elizabethkingia, as well as differentiating the two most commonly reported species, Elizabethkingia anophelis and Elizabethkingia meningoseptica. |
| Databáze: | OpenAIRE |
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