Budding yeast relies on G 1 cyclin specificity to couple cell cycle progression with morphogenetic development
| Autor: | Helen R. Flynn, Frank Uhlmann, Florine Chrétien, Ambrosius P. Snijders, Probir Chakravarty, Deniz Pirincci Ercan |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Multidisciplinary biology Kinase Saccharomyces cerevisiae Cell cycle biology.organism_classification Budding yeast Cell biology 03 medical and health sciences Substrate-level phosphorylation 0302 clinical medicine Cyclin-dependent kinase biology.protein Mitosis 030217 neurology & neurosurgery 030304 developmental biology Cyclin |
| Zdroj: | Science Advances. 7 |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abg0007 |
| Popis: | Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities of successive cyclin waves. Alternatively, in the quantitative model, the gradual rise of Cdk activity from G1 phase to mitosis leads to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in Saccharomyces cerevisiae All S phase and mitotic cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. A single cyclin can also replace all G1 cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. However, single-cyclin cells fail to polarize or grow buds and thus cannot survive. Our results suggest that budding yeast has become dependent on G1 cyclin specificity to couple cell cycle progression to essential morphogenetic events. |
| Databáze: | OpenAIRE |
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