Application of capacitively coupled contactless conductivity as an external detector for zone electrophoresis in poly(dimethylsiloxane) chips
| Autor: | Péter I. Koczka, Ede Bodoki, Attila Gáspár |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Materials science Thermal conductivity detector 010401 analytical chemistry Clinical Biochemistry Microfluidics Detector Analytical chemistry 02 engineering and technology Conductivity 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Electrophoresis Electrode Theoretical plate 0210 nano-technology |
| Zdroj: | ELECTROPHORESIS. 37:398-405 |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201500335 |
| Popis: | In this work, lab-made PDMS microfluidic chips were matched to a capacitively coupled contactless conductivity detector (C(4) D) having external in-plane electrodes (eDAQ, Australia). The advantages of this type of C(4) D are the choice to reversibly place or remove the microchip onto/from the detector and to freely variate the position of the detection (separation length) on the microchip. The thickness of the bottom layer of the PDMS chip was optimized to achieve sensitive detection during the electrophoretic separation. PDMS chips with 100 μm bottom layer used with the C(4) D platform were tested by CZE of a mixture of seven anions and different types of real samples. Using split-flow pressure sample injection and effective length of 6.5 cm, the numbers of theoretical plates were in the range of 4000-6000 (63,000-93,000/m) and the LODs amounted to 3.66-14.7 μmol/L (0.13-2.26 μg/mL) for the studied anions. |
| Databáze: | OpenAIRE |
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