Method for detection of a few pathogenic bacteria and determination of live versus dead cells
| Autor: | James M. Barbaree, Songtao Du, Sang-Jin Suh, Howard C. Wikle, Bryan A. Chin, I-Hsuan Chen, Shin Horikawa, Yuzhe Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
010302 applied physics
Salmonella biology Pathogenic bacteria 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease_cause biology.organism_classification 01 natural sciences Microbiology Bacteriophage Resonator 0103 physical sciences medicine Biophysics 0210 nano-technology Biosensor Pathogen Nutrient broth Bacteria |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2228142 |
| Popis: | This paper presents a method for detection of a few pathogenic bacteria and determination of live versus dead cells. The method combines wireless phage-coated magnetoelastic (ME) biosensors and a surface-scanning dectector, enabling real-time monitoring of the growth of specific bacteria in a nutrient broth. The ME biosensor used in this investigation is composed of a strip-shaped ME resonator upon which an engineered bacteriophage is coated to capture a pathogen of interest. E2 phage with high binding affinity for Salmonella Typhimurium was used as a model study. The specificity of E2 phage has been reported to be 1 in 105 background bacteria. The phage-coated ME biosensors were first exposed to a low-concentration Salmonella suspension to capture roughly 300 cells on the sensor surface. When the growth of Salmonella in the broth occurs, the mass of the biosensor increases, which results in a decrease in the biosensor's resonant frequency. Monitoring of this mass- induced resonant frequency change allows for real-time detection of the presence of Salmonella. Detection of a few bacteria is also possible by growing them to a sufficient number. The surface-scanning detector was used to measure resonant frequency changes of 25 biosensors sequentially in an automated manner as a function of time. This methodology offers direct, real-time detection, quantification, and viability determination of specific bacteria. The rate of the sensor's resonant frequency change was found to be largely dependent on the number of initially bound cells and the efficiency of cell growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|